This kit follows the standard method ISO13136, and the methodology defined in the EU Reference Laboratory for E. coli EU
RL Method_food_2 REV.2. The method uses the Real time PCR technique to detect the presence of the genes associated with
six non-O157 serotypes – O26, O45, O103, O111, O121, and O145.
The method is applicable to products intended for human consumption and the feeding of animals, environmental samples in the
area of food production and food handling, and environmental samples in the area of primary production. Amplification detection
is based on Taqman technology, i.e., the fluorescence issued is detected upon degradation of the hydrolysis probe during PCR