Shiga toxin-producing E. coli (STEC) also known as Verocytotoxin-producing E. coli (VTEC) are pathogenic E. coli characterized by their ability to produce unique virulence factors (toxins and adhesion molecules). The standardized ISO/TS 13136 method describes the recommendations for the detection of STEC E. coli. The method uses the Real time PCR technique to detect the presence of the stx1 and stx2 genes (associated with cytotoxicity) and the eae gene associated with the capability of the bacteria to adhere to the intestinal epithelium and cause disease.
The method is applicable to products intended for human consumption and the feeding of animals, environmental samples in the area of food production and food handling, and environmental samples in the area of primary production. Amplification detection is based on Taqman technology, i.e., the fluorescence issued is detected upon degradation of the hydrolysis probe during PCR reaction. This kit includes an Internal Amplification Control (IAC) detection primer/probe set as a process control for the presence of PCR inhibitors.