Description
ALScreen – Meat ID Swine Halal provides a Real-Time PCR method for detecting Swine (Sus scrofa) mitochondrial DNA in food,
feed and environmental samples with a remarkable sensitivity, providing a limit of detection of 0.0001%. The method relies on the
amplification of species-specific mitochondrial DNA. This was found to be an advantageous approach as mitochondrial genes are
present in multi-copies, leading to higher detection sensitivity in comparison to single copy target genes found in the nuclear
genome. Amplification detection is based on Taqman technology, i.e., the fluorescence emission is detected upon degradation of
the hydrolysis probe during PCR reaction.