Description
ALScreen – Meat ID Bovine provides a Real-Time PCR method for detecting Bovine (Bos taurus) mitochondrial DNA in food, feed and environmental samples. The method relies on the amplification of species-specific mitochondrial DNA. This was found to be an advantageous approach as mitochondrial genes are present in multi-copies, leading to higher detection sensitivity in comparison to single copy target genes found in the nuclear genome. Amplification detection is based on Taqman technology, i.e., the fluorescence emission is detected upon degradation of the hydrolysis probe during PCR reaction.